- 询价
- Cell Signaling Technology
- 3270
- USA
- 2025年08月16日
- W, IP, IHC-P, IF-IC, F
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-c-Jun (Ser73) (D47G9) XP Rabbit mAb__®
- 抗原：
synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk | Endogenous | 48 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb detects endogenous levels of c-Jun only when phosphorylated at Ser73. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun. Western Blotting
Western blot analysis of extracts from NIH/3T3 or C6 cells, untreated or UV-treated, using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of parafin-embedded human colon carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser73) Blocking Peptide (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb. Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or UV treated (green), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). |
| Background | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
过程中，一抗应在封闭缓冲液 (TBS/0.3%Triton. X–100/5% NGS) 中稀释。抗体：Phospho–Akt (Ser473) (D9E) XP. Rabbit mAb #4060、Phospho–EGFReceptor (Tyr1173) (53A5) Rabbit mAb #4407样本：石蜡包埋人类乳腺肿瘤（上图）和 HCC827 异种移植物（下图）抗体稀释：SignalStain. 抗体稀释液（左）或 TBST/5%NGS（右）检测—检测系统传统的 IHC 检测
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