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- prospec
- COL20-150
- 以色列
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
20 well comb
- 供应商：
北京鼎国昌盛
- 库存：
大量
- 规格：
1
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文献和实验SDS-PAGE Western Blotting Protocols
liquid. 11. Pour stacking gel to fill the remaining space between the glass plates. 12. Insert comb and let polymerize (this takes ~10-15’). 13. Prepare 20-30ml of sample, as follows: 14. Boil samples for at least 3’. 15. Place gel
in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes
with a pipette. Insert the teeth of the comb approximately 1 mm into the gel. Pre-heat the gel at 1700 volts for 15-20 minutes. F: Gel Loading Remove the samples from the freezer. Denature the samples in a thermal cycler at 95°C for 5 mins. Remove
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