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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
IEF Ampholyte Mix
- 规格:
1
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文献和实验Advantages and Disadvantages of Carrier Ampholyte IEF
There are several advantages to the use of carrier ampholytes for running IEF separations. Tube gels using carrier ampholytes are easy to prepare and do not require sophisticated gradient casting equipment. Ampholyte mixtures can be simply
Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D
2-D gel electrophoresis is one of the most effective techniques for high-resolution separation of complex protein mixtures. Recent developments in the field of protein mass spectrometry allow the rapid and highly sensitive identification
Protein extraction from whole tissues for IEF
in a fume hood. Transfer to a 15 ml Falcon tube and add 2.5 ml of Tris pH8.8 buffered phenol and mix. Sonicate for 30 s at setting 60 and then agitate for 30 min at 4 C. Centrifuge 10 min at 6000 rpm (SS-34), 4 C. Remove 1 ml of the phenol
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