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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京鼎国昌盛
- 英文名：
12 x 10.2 cm notched alumina plate
- 规格：
5
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
EDTA 9. Leave at room temperature or at 30 for 24-48 hrs. 10. Collect the cells by centrifuging at 16,000 x g for 30 sec. 11. Aspirate the supernatant. 12. Resuspend the cells in 200 ml of sterile water by pipetting gently
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