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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
北京鼎国昌盛
- 库存:
大量
- 英文名:
Short and long plates, 5 mm thick, 5 sets
- 规格:
10
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文献和实验interfering double-stranded RNAs (siRNAs) need to be applied. This protocol describes a straightforward, low-cost method, which uses recombinant Escherichia coli RNase III to digest long dsRNA into endoribonuclease-prepared short interfering RNAs (esiRNAs
RNase Protection Assay(RPA)操作步骤
by ethanol. Siliconize the short plate and clean again. Assemble the gel mold (0.4 mm spacers). 22.Combine the following to give a final concentration of 5% acrylamide: 74.5 ml acrylamide solution (final 19:1 acrylamide/bis): 8.85 mls of 40
(5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’), 0.30 mM dNTPs, 1.0 mM MgSO4, 5 U Platinum® Pfx Polymerase (Invitrogen), PCR Enhancer(Invitrogen) and Pfx Amplification Buffer (Invitrogen). 30 cycles of PCRs are run with the following cycling program: 94 ℃
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