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- prospec
- PKB4.0
- 以色列
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京鼎国昌盛
- 英文名：
GeneCatcher disposable gel excision tips, 4.0 mm x 1.0 mm
- 库存：
大量
- 规格：
250
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文献和实验Radiolabeled sequencing gel preparation, loading, and electrophoresis
terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA. The reactions terminated with the long termination mix typically
In-gel digestion of proteins for peptide fingerprint mapping
Dissolve trypsin just before use in ice-cold buffer (to reduce auto-proteolysis). 1. Excise protein bands/spots of interest from the gel and cut each gel piece into small particles (~1 mm x 1 mm) using a scalpel and place into a 0.65 ml
SSR GEL and Silver Staining Protocol
gloves when handling solution and face mask when weighing out powder. A safer alternative is to buy a premix. 1. Rinse all glassware and plastic ware with d.i. water prior to gel solution preparation and pouring, including the disposable filter unit
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