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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Formamide, deionized, sequencing grade
- 供应商：
北京鼎国昌盛
- 库存：
大量
- 规格：
1
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文献和实验Direct PCR Sequencing with Denaturants (Formamide)
The advance of Taq -based polymerase chain reaction (PCR) technology (1 –3 ) has had a tremendously positive impact on biomedical research. The combination of PCR and sequencing further revolutionized biological research
(Pharmacia) Stop solution (95% deionized formamide, 20mM EDTA, pH 7.5, 0.1% each of bromophenol blue and xylene cyanol FF) 1) Denature dsDNA by the addition of 8ul DNA (approximately 3ug) to a sterile microcentrifuge tube containing 2ul freshly made 2M
Inverse PCR & Cycle Sequencing
(Deionized formamide) to 1 part (25 mM EDTA; 50 mg/ml Blue Dextran) 5) Load 1.9 ul onto gel Pharmacia ALF Sequencers have also been used for direct cycle sequencing of PCR products using dye primer technology. Good results were obtained using
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