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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Water, deionized, Dnase & Rnase free, sequencing grade
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 规格:
1
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文献和实验,000 x G for 1 minute 2) For each HiBind® RNA Minicolumn, prepare the DNase I digestion reaction mix as follows: OBI DNase I Digestion Buffer 73.5ul RNase-free DNase I (20 Kunitz unites/ul) 1.5ul Total volume 75ul
How to Maintain an RNase-free Lab
transcription and RT-PCR Weekly Thoroughly clean lab benchtops, pipettors (see sidebar below) and tube racks with RNaseZap ® Monthly Test water source for RNase using RNaseAlert - distilled deionized or ultrapure water is often RNase
to hydrolyze DNA, using an RNAse free DNAseI, and purify the cleaned RNA free of DNA and free of DNAseI activity.ProcedureTransfer the total RNA to a clean 1.5 ml tube and adjust the volume of the total RNA sample to 100 ul with RNAse free water.Add 350 ul
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