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- 文献和实验
- 技术资料
- 英文名:
Water, deionized, Dnase & Rnase free, sequencing grade
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 规格:
1
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文献和实验How to Maintain an RNase-free Lab
transcription and RT-PCR Weekly Thoroughly clean lab benchtops, pipettors (see sidebar below) and tube racks with RNaseZap ® Monthly Test water source for RNase using RNaseAlert - distilled deionized or ultrapure water is often RNase
,000 x G for 1 minute 2) For each HiBind® RNA Minicolumn, prepare the DNase I digestion reaction mix as follows: OBI DNase I Digestion Buffer 73.5ul RNase-free DNase I (20 Kunitz unites/ul) 1.5ul Total volume 75ul
to hydrolyze DNA, using an RNAse free DNAseI, and purify the cleaned RNA free of DNA and free of DNAseI activity.ProcedureTransfer the total RNA to a clean 1.5 ml tube and adjust the volume of the total RNA sample to 100 ul with RNAse free water.Add 350 ul
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