BetterBuffer BigDye™ dilution

蛋白偶联到磁性MagPlex™微球的方法

microspheres = count (1 corner of 4 x 4 section) x (1 x 104 ) x (dilution factor) x (resuspension volume in mL) 31.Store coupled microspheres refrigerated at 2-8°C in the dark. Technical Note 1 : For a list of magnetic separators, see Recommended

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume:       1.5 ml 1M Hepes       1.05ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       24.15ml H20 ChIP high salt buffer       30ml final volume:       1.5ml 1M Hepes