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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
200ul filtered tips
- 规格:
1
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
设备 Equipments supplied by User 1. Magnetic separation device for 96 well microplate (MSD-01) 2. Nuclease-free 96-well 3. Multichannel pipette 4. Nuclease-Free pipetting tips 实验步骤 The following protocol
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
instead of TE since the EDTA inhibits Taq Polymerase. 12、The DNA concentration then is estimated by analysis of 10 ul of the 210 ul final DNA solution by agarose gel electrophoresis and by measuring the A260 in the spectrometer. Typical BAC yields per 200 ml
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