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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
北京鼎国昌盛
- 库存:
大量
- 英文名:
5 lane comb, 0.75 mm thick
- 规格:
1
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文献和实验【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
2.1. 3' RACE LaNe 1. 20 ng total RNA (see Note 1). 2. 10 mM dNTPs. 3. ThermoScript ® Reverse Transcriptase and accompanyingbuffers (Invitrogen) (see Note 2). 4. TTT3 primer: 5'-gene-specific sequence followed byTTTTTTTTTTTTTTTTV
Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
dryer (Life Technologies)Glass plates, 31.0 x 38.5 cm (Life Technologies)0.4 mm spacers (Life Technologies)Casting boot (Life Technologies)Shark tooth comb (Life Technologies)Small clampsWhatman blotting paper, 3 mm thicknessKodak Biomax MR or AR
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