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- 2026年02月07日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Bisulfite-Converted Universal Methylated Human DNA Standard
- 供应商：
简石生物
HIGHLIGHTS
- DNA that has been enzymatically methylated at all cytosines within a CpG dinucleotide context and bisulfite-converted for direct use in downstream methylation applications.
- Provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
DESCRIPTION
The Bisulfite-Converted Universal Methylated Human DNA Standard is designed to be used as a control for DNA bisulfite conversion and downstream analyses including PCR, MSP, and other amplification-based assays. This DNA is identical to our Universal Methylated Human DNA Standard, but has been bisulfite-converted using the EZ DNA Methylation – Direct (D5020) kit. This product is provided as a 20 ng/µl solution and contains enough for 50 reactions. The primer set included with the standard has been designed and validated to amplify a segment of the bisulfite-converted DNA.
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文献和实验相关专题 Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing
need to be even more sensitive due to the reduced form of the DNA. When DNA is converted using bisulfite, the non-methylated Cs are converted to Ts while the methylated Cs remain the same. Therefore, using standard sequencing equipment the detection of a C indicates
Bisulfite Modification for Analysis of DNA Methylation
an important role in the control of gene function. This procedure, called bisulfite genomic sequencing, is a principal method for the analysis of DNA methylation in various biological phenomena, including human diseases such as cancer. This unit describes
