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- 2026年01月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 英文名：
ChIP DNA Clean & Concentrator
- 供应商：
简石生物
HIGHLIGHTS
- Two minute DNA clean-up from any step in a standard ChIP protocol.
- DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.
DESCRIPTION
The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high-quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP.
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文献和实验糖凝胶上检测分析。如图一所示 图一：2.2 超声破碎后，8000g，30秒，4°C,离心。将上清移入新的管子中。开始准备进行染色质免疫共沉淀（IP）。2.3 每份超声样品取50ul，这时的样品标记为INPUT，用于定量DNA浓度和作为PCR的空白对照。超声后的染色质应立刻冻入液氮中或者储存于-70°C可储存2个月。避免多次反复冻融。3检测DNA浓度1INPUT样品用于为后来的IP样品计算DNA浓度。DNA可采用PCR纯化试剂盒（加入70ul洗脱液，接着进入3.2a）或者采用酚/氯仿抽提（加入350ul
for downstream digests oheat 5 minutes at 60 degrees ospin 2 min @ full speed in microcentrifuge oremove DNA-containing supernatant to clean tube Notes & Misc: •Conveinient PCR clean up before digestion: 100μl PCR reaction cleaned up with 50μl silica
Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
