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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 英文名:
ZymoPURE II Plasmid Gigaprep Kit
- 供应商:
简石生物
HIGHLIGHTS
- Fast: 50 minute Gigapreps
- Highest Yield: Purify up to 10 mg of plasmid DNA directly from a spin-column.
- Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
DESCRIPTION
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 10 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Gigaprep kit can process up to 2.5 L of culture in less than 50 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with a vacuum instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl) , EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Gigaprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
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文献和实验问:我欲提酵母质粒。我在为酵母细胞破碎犯愁,有人说超声波就可以。但大多人说对于酵母不行,高手们请指教了!谢了!答:军科院的方法:1、用酵母细胞裂解液重悬酵母菌2、加酚-氯仿3、加玻璃珠4、振荡半小时5、乙醇沉淀6、电转大肠杆菌Detailed:Plasmid Isolation from YeastReagents and Materials RequiredThe YEASTMAKER Yeast Plasmid Isolation Kit (#K1611-1) provides
相关专题 问:我欲提酵母质粒 。我在为酵母细胞破碎犯愁,有人说超声波就可以。但大多人说对于酵母不行,高手们请指教了!谢了! 答:军科院的方法: 1、用酵母细胞裂解液重悬酵母菌 2、加酚-氯仿 3、加玻璃珠 4、振荡半小时 5、乙醇沉淀 6、电转大肠杆菌 Detailed: Plasmid Isolation
质粒提取过程参照E.Z.N.A Plasmid Extraction Kit进行。 (1) 用无菌牙签挑取平板上白色单菌落(5~10个)分别接种至3-5ml LB培养液(Amp )中,200rpm培养14~16小时; (2) 室温下,10,000g离心1分钟收菌; (3) 加入溶液I 250μl,涡旋重悬菌体; (4) 加入溶液II 250μl,温和倒转4~6次混匀,室温放置2分钟 (5) 加入350μl溶液III,温和倒转至有白色絮状沉淀产生, 室温下,10











