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- 英文名：
Quick-DNA Plant/Seed Miniprep Kit
- 供应商：
简石生物
HIGHLIGHTS
- Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
DESCRIPTION
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
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文献和实验以下操作按照天根产品 DP321 快捷型植物基因组 DNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 植物叶片2. 研钵 液氮3. 移液器及配套无菌枪头（200 μl ，1ml），1.5 ml离心管4. 异丙醇，70%乙醇，干净吸水纸5. 涡旋振荡器，金属浴/水浴，台式离心机本文版权归天根生化科技（北京）有限公司所有,仅供个人学习使用,请勿转载
实验步骤 1. Protocol for grinding plant seeds. 1) grind using a bead mill a. place one seed into each well of a 2 ml square well block. b. Pipette nuclease-free water
Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992) Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
