- ￥3870
- Sino Biological
- 80231-R09E
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：Galectin 8/LGALS8蛋白, Galectin 8/LGALS8 protein
蛋白构建：A DNA sequence encoding rat LGALS8 (NP_446314.2) (Met 1-Trp 316) was fused with the GST tag at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.05-0.3 μg/mL.
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant rat LGALS8/GST chimera consists of 548 amino acids and has a predicted molecular mass of 63.2 kDa. It migrates as a 55KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_446314.2
蛋白氨基酸序列：Met1-Trp316
蛋白标签：N-GST
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验kinases activate the serine/threonine kinases M n k l and M n k 2 . EMBO J. 16, 1909-1920. 13. Fujita, H., Fujita, H., Takemura, M., et al. (2003) A n ArabidopsisM A D S - b o x protein, A G L 2 4 , is specifically bound to and phosphorylated
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
蛋白质定量分析(Protein determination)
1. 每槽先加好50 μL标准品 (#0, #1 … #5) 或未知样本 (X1, X2)，以二重复加入96 孔滴定盘中，如图所示。 微量滴定盘的使用 2. 每槽再加入200 μL Dye-Reagent (d)，在全部样本槽加完前，不要中断添加；同时小心避免气泡产生。 3. 轻拍滴定盘一侧，使添加的各成份均匀混合，注意Dye-Reagent 密度较大，很容易沉积在下方而分层
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