- ￥2310 - 15050
- Sino Biological
- 北京
- 50654-M08B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse Notch-1 / NOTCH1 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50.00 µg/100.00 µg/500.00 µg
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 500.00 µg | 产品价格： | ￥15050.0 |
蛋白名称：NOTCH1蛋白, NOTCH1 protein
蛋白构建：A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 82 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his (Cat:50640-M08H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 19
蛋白分子量：The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_032740.3
蛋白氨基酸序列：Met1-Gln526
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
‐affinity tag antibodies for development and determination of protein display, 2624B (Cell Signaling Technology) Hybridization chamber (Corning, product no. 2551
Transgene Detection Assays: Validation and Protocols
fragment from the mouse Notch1 gene using these PCR reaction conditions. N1.1: 5' GAT ATC GTG GTG CAT ACC CTC CTG 3' N1.2: 5' GTG GTC TAG GAT GCT TGG GTC TAG 3' The number of cycles should be no more than 30-40, and should be performed on 0.2
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