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- 规格:
50 µg
蛋白名称:ERK2蛋白, ERK2 protein
蛋白构建:A DNA sequence encoding the human ERK2 (NP_002736.3) (Met 1-Ser 360) was fused with the GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:No Kinase Activity
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human ERK2/GST chimera consists of 585 amino acids and predicts a molecular mass of 67 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号:NP_002736.3
蛋白氨基酸序列:Met1-Ser360
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验MAP-kinase antibody and anti-Dictyostelium ERK2 antibody. In this chapter, we describe the methods we have used to assess the level of activated ERK2 and partial involvement of G protein in cAMP-induced ERK2 activation.
yirenzhe ERK1与ERK2到底有什么区别?除分子量不同外。 doctormy ERK1 和 ERK2 的区别是个很大的研究课题,还有很多未知啊,呵呵 目前研究认为,ERK1 和ERK2 的上游激活路径高度相似,而且他们目前已知的下游靶点也非常一致。但当前有关区别的研究主要还是侧重于具体的生物学功能方面:人们发现ERK2与人体发育密切相关,对于学习和记忆的功能非常重要,而ERK1则不然;有学者认为
Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
to be CRM1- and MEK1/2-dependent. Here, we describe a general nuclear import assay of wild-type ERK2 that can be employed to identify different mechanisms governing nuclear entry of the protein kinase, adapted to evaluate ERK2 mutants that impair nuclear
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