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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse ITGAV & ITGB6 Heterodimer Protein (Flag & His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥5170 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥33640 |
蛋白名称:Integrin alpha V beta 6蛋白, Integrin alpha V beta 6 protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Val988) of mouse ITGAV (NP_032428.2) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Pro708) of mouse ITGB6 (NP_067334.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse ITGAV&ITGB6 heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 31 & Gly 22
蛋白分子量:The recombinant heterodimer of mouse ITGAV&ITGB6 comprises 1728 (1000+728) amino acids and has a calculated molecular mass of 190.4 (110.7+79.7) KDa.
蛋白NP号:NP_032428.2 & NP_067334.1
蛋白氨基酸序列:Met1-Val988 & Met1-Pro708
蛋白标签:C-Flag & C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Arabidopsis protein microarrays. Proc. Natl. Acad. Sci. U.S.A. 104:4730‐4735. Ptacek, J., Devgan, G., Michaud, G., Zhu, H., Zhu, X., Fasolo, J., Guo, H., Jona, G., Breitkreutz
Mouse Strains and Genetic Nomenclature
., and Montagutelli, X. 2009. Genetic analysis of skull shape variation and morphological integration in the mouse using interspecific recombinant congenic strains between C57BL/6 and mice of the Mus spretus species. Evolution 63:2668‐2686
Methods to Detect Protein Glutathionylation
of Contents Introduction Basic Protocol 1: Glutathionylation of Recombinant Protein and Detection by Immunoblotting Basic Protocol 2: Detection
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