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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Semaphorin 3A / SEMA3A Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/100.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥2310.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥5820.0 |
蛋白名称:SEMA3A蛋白, SEMA3A protein
蛋白构建:A DNA sequence encoding the mouse SEMA3A (NP_006071.1) (Lys26-Phe546) was expressed with a polyhistidine tag at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:His
蛋白分子量:The recombinant mouse SEMA3A consists 540 amino acids and predicts a molecular mass of 61.6 kDa.
蛋白NP号:NP_006071.1
蛋白氨基酸序列:Lys26-Phe546
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture
We examined the role of semaphorin3a in ureteric bud (UB) branching morphogenesis using mouse metanephric organ culture [Tufro et al. (Mech Dev 125:558–568, 2008)]. In vitro UB injection of Sema3a antisense morpholino resulted in increased
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
serumfreeconditioned medium (11) ). 3. Methods When expressing recombinant proteins, the choice of vector hasa major impact on purification strategy, yield, and utility of theproduct. Vectors that link a poly-histidine tag to the recombinant protein typically
Studies of the Ubiquitin Proteasome System
of Wheat E1 in E. coli PET3a, pET15b or other vector for expression of proteins in E. coli from a T7 promoter but without a protein sequence Basic Protocol 21: Preparation of Mouse E1 in Insect Cells Using a Baculovirus
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