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200 µg
蛋白名称:Frizzled 2蛋白, Frizzled 2 protein
蛋白构建:A DNA sequence encoding the mouse Fzd2 (NP_065256.1) (Gln29-Leu168) was expressed with the Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Glu
蛋白分子量:The recombinant mouse Fzd2 consists of 400 amino acids and predicts a molecular mass of 44.2 kDa.
蛋白NP号:NP_065256.1
蛋白氨基酸序列:Gln29-Leu168
蛋白标签:N-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验液。用ProteoExtract TM-PEK试剂抽提得到的蛋白适合用于常见的各种蛋白分析方法。从以下的报告中,列举了TM-PEK制备的跨膜蛋白和多次跨膜蛋白样品在免疫印迹、活性分析及2D电泳等方面的实例。为了验证ProteoExtract跨膜蛋白抽提试剂盒抽提多次跨膜蛋白的有效性,我们用免疫印迹比较了Frizzled-4,CELSR-3(cadherin-EGF-lag seven-pass receptor-3)和EGFR(表皮生长因子,只有一个跨膜区)的样本制备效果。Frizzled和CELSR是WNT/ PCP
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
with the Wnt preparation and cellular context. Key words: sFRP , Recombinant expression , Protein purification , Heparin chromatography , Chelating chromatography , Wnt 1. Introduction Secreted Frizzled-related proteins (sFRPs) contain a Frizzled(Fzd
Background Information of RNAi
. Much of this response is due to activation of the dsRNA -dependent protein kinase PKR, and the subsequent phosphorylation and inactivation of the translation factor eIF2a. As RNA i became better understood, scientists discovered that double stranded short interfering
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