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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human LY75 / DEC-205 / CD205 Protein (ECD,His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥4520.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥29480.0 |
蛋白名称:Human LY75 / DEC-205 / CD205 Protein (ECD,His Tag)
蛋白构建:A DNA sequence encoding the human LY75 (NP_002340.2) (Ala32-Gly1664) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 32
蛋白分子量:The recombinant human LY75 consists 1644 amino acids and predicts a molecular mass of 189.7 kDa.
蛋白NP号:NP_002340.2
蛋白氨基酸序列:Ala32-Gly1664
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验上 Strep-tag 的 Fab 片段(Fab-Streps)特异性的结合到琼脂糖基质上。随后,加入全血或其他血液制剂流过柱子。目的细胞通过特异性的 Fab-Strep 作用从而结合到琼脂糖基质上,其他非目的细胞则有效的被洗去。最后,加入生物素使目的细胞从 Fab-Streps 上解离。至此,就已分离得到不带任何标记的、真实的目的细胞可用于下游的实验研究。 1. 实验流程 所需产品: CD3 Fab-TACS® Gravity Kit, human(IBA Lifesciences, Cat
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
Dynabeads and cells, the incubation temperature must be 2-8°C to reduce phagocytic activity and other metabolic processes. 3. Never use less than 25 μl (1 x 107) Dynabeads per ml of cell sample and at least 4 Dynabeads per target cell.
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
5:135‐146. Hu, S., Xie, Z., Onishi, A., Yu, X., Jiang, L., Lin, J., Rho, H.S., Woodard, C., Wang, H., Jeong, J.S., Long, S., He, X., Wade, H., Blackshaw, S., Qian, J., and Zhu
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