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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human / Rhesus / Cynomolgus / Canine TGF-beta 1 / TGFB1 Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥37960.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥680.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1980.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4980.0 |
| 规格: | 500.00 µg | 产品价格: | ¥16180.0 |
蛋白名称:Human / Rhesus / Cynomolgus / Canine TGF-beta 1 / TGFB1 Protein
蛋白构建:A DNA sequence encoding the active form of human / rhesus / cynomolgus / canine TGFβ1 (NP_000651.3) (Ala 279-Ser 390) was expressed and purified. Human, Rhesus, cynomolgus and Canine TGFβ1 sequences are identical.
表达宿主:CHO Stable Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.04-0.2 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 279
蛋白分子量:The recombinant human / rhesus / cynomolgus / canine TGFβ1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
蛋白NP号:NP_000651.3
蛋白氨基酸序列:Ala279-Ser390
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Zhang, H; et al. Alogliptin alleviates liver fibrosis via suppression of activated hepatic stellate cell. Biochemical and biophysical research communications, PMID: 30797555
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,来自美国宾夕法尼亚大学和俄亥俄州立大学的研究团队通过非人灵长类动物 (NHP) 筛选,成功开发出 AAV-EP+衣 壳变体,对室管膜细胞的转导效率比其亲本 AAV1 高 22 倍,能够广泛转导大脑皮质神经元和深层脑区,在整个脑室系统中实现 均匀分布。AAV-EP+在三种非人灵长类动物(恒河猴、非洲绿猴、食蟹猴)、两种小鼠品系(C57BL/6 和 FVB)和人源化诱导多能干 细胞(iPSC)衍生神经元中都表现优异。在 NHP 中,AAV-EP+的使用剂量比以往报道低 30 倍以上,达到的蛋白
白细胞介素-2(interleukin rhlL-2,IL-2)主要由活化的T淋巴细胞分泌的一种糖蛋白,是调节机体免疫功能最主要的淋巴因子之一。天然IL-2含有糖基,基因重组IL-2不含糖基,由133个氨基酸组成,相对分子质量为15KD。IL-2在机体应答,免疫调节和抗肿瘤免疫中具有十分重要的作用。临床用于多种恶性实体肿瘤。重组人IL-2是一种重组人白细胞介素-2的生物制品,一般制备成粉针剂应用,其规格以活性单位而不是以重量单位标示。本研究拟通过双抗体放射免疫分析法又称为免疫沉淀法测定注射
AKT的实验时,是不是要加TGF-beta, 药物,IGF-1三种东西到你的细胞中,会不会太复杂了。建议你能够和别人要到组成型活性的AKT的表达载体,转入到你的细胞中,这样分析可能比较好。 个人观点,仅供参考。 fghostyun 你的这个设计是没有问题的。但是你的PI3K/AKT指标实际上需要测两种:一个是PI3K/AKT的蛋白表达量, 一个是PI3K/AKT的蛋白激酶活性(磷酸化与被磷酸化)。 此外,我看你是打算药物刺激作用后提取PI
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