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100 µg
蛋白名称:Interferon alpha-B蛋白, Interferon alpha-B protein
蛋白构建:A DNA sequence encoding the cynomolgus IFNA8 (G7PS89) (Met1-Glu189) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 1-5 pg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 33
蛋白分子量:The recombinant cynomolgus IFNA8 comprises 177 amino acids and has a calculated molecular mass of 21 KDa. The apparent molecular mass of it is approximately 33 KDa respectively in SDS-PAGE.
蛋白NP号:G7PS89
蛋白氨基酸序列:Met1-Glu189
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验【求助】哪里能买到含有人干扰素alpha-2b cDNA序列的质粒pBV889
baiguo861029 各位前辈好 本人是个新手,听很多师兄师姐说丁香园很好,这儿的人都很热情帮助他人。我现在研究生在读,因为课题需要,急需购买这个质粒pBV889,试了好多方法都没找到。这个质粒含有人干扰素alpha-2b编码序列,即cDNA序列。非常希望知道的前辈给予我帮助,非常感谢!! woxingwosu 用google搜索了一下,发现很多文章都有,国内发表的也很多。其实你或者你老板可以写信去要一下就方便多了,还不用
Measuring Interferon Alpha and Other Cytokines in SLE
The progression of disease in patients with systemic lupus erythematosus (SLE) is affected by production, accumulation, and actions of cytokines. Type I interferon (IFN), specifically IFN-α, is recognized as a central mediator of disease
Protein Engineering of Interferon Alphas
Interferon (IFN)-αs constitute a family of proteins exhibiting high degree of homology in primary, secondary, and tertiary structure and display a high level of species specificity in their biological properties. However, small structural
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