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100 µg
大鼠 Frizzled 4蛋白|Frizzled 4 protein|Frizzled 4(Rat, C-human IgG1-Fc)(产品说明)
蛋白名称:Frizzled 4蛋白, Frizzled 4 protein
蛋白构建:A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 38
蛋白分子量:The recombinant rat FZD4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q9QZH0
蛋白氨基酸序列:Met1-Glu181
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称:Frizzled 4蛋白, Frizzled 4 protein
蛋白构建:A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 38
蛋白分子量:The recombinant rat FZD4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q9QZH0
蛋白氨基酸序列:Met1-Glu181
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验相关实验
液。用ProteoExtract TM-PEK试剂抽提得到的蛋白适合用于常见的各种蛋白分析方法。从以下的报告中,列举了TM-PEK制备的跨膜蛋白和多次跨膜蛋白样品在免疫印迹、活性分析及2D电泳等方面的实例。为了验证ProteoExtract跨膜蛋白抽提试剂盒抽提多次跨膜蛋白的有效性,我们用免疫印迹比较了Frizzled-4,CELSR-3(cadherin-EGF-lag seven-pass receptor-3)和EGFR(表皮生长因子,只有一个跨膜区)的样本制备效果。Frizzled和CELSR是WNT/ PCP
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
with the Wnt preparation and cellular context. Key words: sFRP , Recombinant expression , Protein purification , Heparin chromatography , Chelating chromatography , Wnt 1. Introduction Secreted Frizzled-related proteins (sFRPs) contain a Frizzled(Fzd
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Frizzled 4 蛋白|Frizzled 4 protein|Frizzled 4(Rat, Fc Tag)
¥3870





