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- Sino Biological
- 北京
- 50038-M08H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse HGF / Hepatocyte Growth Factor Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/100.00 µg/1.00 mg
| 规格： | 20.00 µg | 产品价格： | ￥1580.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥4520.0 |
| 规格： | 1.00 mg | 产品价格： | ￥19800.0 |
蛋白名称：HGF蛋白, HGF protein
蛋白构建：A DNA sequence encoding the mouse HGF (Q08048-1) (Met1-Leu728) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 70 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 1-8 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 33
蛋白分子量：The recombinant mouse HGF comprises 707 amino acids and has a predicted molecular mass of 80.8 kDa. The apparent molecular mass of the protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：Q08048-1
蛋白氨基酸序列：Met1-Leu728
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Hepatocyte Growth Factor Gene Therapy for Hypertension
Hepatocyte growth factor (HGF) has mitogenic, motogenic, and morphogenic biological activities as well as helps in regenerating various tissues. In cardiovascular organs, HGF was reported to have anti-apoptotic, anti-fibrotic
His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
HGF-Induced DU145 Cell Scatter Assay
to be adopted by some invasive cancer cells. EMT requires modifications in cell shape and substratum adhesions and these events are dependent on the reorganisation of the actin cytoskeleton. Hepatocyte growth factor (HGF) is a mitogenic growth factor
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