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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse CD4 Protein (ECD, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥25190.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:CD4蛋白, CD4 protein
蛋白构建:A DNA sequence encoding the extracellular domain of mouse CD4 (NP_038516.1) (Met 1-Thr 394) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well are added to mCD4-His coated plates (0.8μg/mL and 100μL/well), approximately >50% will adhere specifically after 30 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Lys 27
蛋白分子量:The recombinant mouse CD4 consists of 379 amino acids and has a predicted molecular mass of 42.8 kDa. As a result of glycosylation, the apparent molecular mass of rmCD4 is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_038516.1
蛋白氨基酸序列:Met1-Thr394
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Th17 Polarization of Mouse CD4 Cells
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免疫亲和层析法常用于蛋白质等生物大分子的纯化。但你是否想过,一根亲和层析柱子也能用来进行细胞分选,而且还不使用高亲和力抗体? 在细胞分选中,我们常常利用免疫识别特性来进行,比如流式分选(FACS)和免疫磁分选(MACS)。 免疫细胞的划分是依据表面标志 CD 分子,可以被抗体识别。比如针对 CD4 + T 细胞这一特定类群,表面含有 CD45、CD3 和 CD4 标记。而表达 CD4 就是辅助性 T 细胞的特征。 由此而来,免疫磁分选利用特定抗体偶联磁珠来捕获细胞。细胞与磁珠
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
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