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100 µg
蛋白名称:JAM-2/JAM-B蛋白, JAM-2/JAM-B protein
蛋白构建:A DNA sequence encoding the rat JAM2 (Q3MHC0) (Met1-Asn236) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When cells (8 x 104 cells/well) are added to rat JAM2-His coated plates (5 μg/mL, 100 μL/well), >30% will adhere after 60 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 29
蛋白分子量:The recombinant rat JAM2 comprises 219 amino acids and predicts a molecular mass of 24.7 kDa. The apparent molecular mass of the recombinant protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:Q3MHC0
蛋白氨基酸序列:Met1-Asn236
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting
The genome of hepatitis B virus (HBV) is a partially double-stranded DNA molecule within virus particles that is approx 3.2 kbp long (1 ,2 ). It has four open reading frames, all on one strand, that encode the surface antigen or envelope
Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition
CD2 is a cell adhesion molecule that mediates T-cell activation by binding to its ligand CD58 on antigen-presenting cells. Interaction between CD2 and CD58 or leukocyte function-associated antigen-3 (LFA-3) helps to optimize immune
Measurement of Cell Adhesion Under Static Conditions
Materials: Static cell adhesion Wash Chamber Test cells in suspension Test cell adhesion molecule 6-carboxyfluorescein diacetate Microtiter plate (96-well) Automatic 96-well plate
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