大鼠 TNFRSF1A蛋白|TNFRSF1A protein|TNFRSF1A(Rat, C-human IgG1-Fc)(产品说明)
蛋白名称:TNFRSF1A蛋白, TNFRSF1A protein
蛋白构建:A DNA sequence encoding the rat TNFRSF1A (P22934) (Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα.2. Immobilized mouse TNFa (80-235) (Cat:50349-MNAE ) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ile 22
蛋白分子量:The recombinant rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:P22934
蛋白氨基酸序列:Met1-Ala211
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.