GLA / alpha-Galactosidase A 蛋白|GLA / alpha-Galactosidase A protein|GLA / alpha-Galactosidase A(Mouse, His Tag)
- ￥4520
- Sino Biological
- 50964-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
50 µg
蛋白名称：GLA/alpha-Galactosidase A蛋白, GLA/alpha-Galactosidase A protein
蛋白构建：A DNA sequence encoding the mouse Gla (Q8BGZ6) (Met1-Arg421) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to hydrolyze 4-methylumbelliferyl-α-D-galactopyranoside. The specific activity is > 400 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 34
蛋白分子量：The recombinant mouse Gla comprises 399 amino acids and has a predicted molecular mass of 45.6 kDa. The apparent molecular mass of the protein is approximately 46-52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：Q8BGZ6
蛋白氨基酸序列：Met1-Arg421
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验LABELING ALPHA-ACTININ WITH IATR
with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
., and Nicolas, J.-F. 1987. A ß-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies. Proc. Natl. Acad. Sci. USA 84 :6795. Cotson, S. and S.J. Holt. 1958. Studies in enzyme cytochemistry IV. Kinetics of aerial oxidation
Assay for ß-galactosidase in E. Coli
1. Constitutive - those enzymes that are continuously synthesized in the cell (e.g. the enzymes of glycolysis or the lac repressor protein); 2 Inducible - those enzymes that are synthesized
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