Alkaline phosphatase/APLI重组蛋白|Recombinant Human Alkaline Phosphatase / ALPI Protein (His Tag)
- ￥1790 - 3870
- Sino Biological
- 北京
- 13225-H08H
- 2026年01月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Alkaline Phosphatase / ALPI Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥1790.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Alkaline phosphatase蛋白, Alkaline phosphatase protein
蛋白构建：A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 1,000 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Val 20
蛋白分子量：The secreted recombinant human ALPI consists of 495 amino acids and has a predicted molecular mass of 54 kDa. The apparent molecular mass of rhALPI is approximately 66 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P09923
蛋白氨基酸序列：Met1-Asp503
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Takeuchi, A; et al. Intestinal alkaline phosphatase is a receptor for cholesterol-lowering pentapeptide IIAEK and regulates cholesterol homeostasis in mice. Scientific reports, PMID: 40594298
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
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