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- 2026年01月26日
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50 µg
蛋白名称：Alkaline phosphatase蛋白, Alkaline phosphatase protein
蛋白构建：A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 83 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 10,000 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Val 20
蛋白分子量：The secreted recombinant human ALPI/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 725 amino acids and has a predicted molecular mass of 79.5 kDa. The apparent molecular mass of rhALPI /Fc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P09923
蛋白氨基酸序列：Met1-Asp503
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验in neurons. Human genetic disorders have been identified in five GPI proteins other than prion These include Marfan's syndrome [FBN1 gene] and Charcot-Leyden crystals [LPPL gene], alkaline phosphatase: infantile hypophosphatasia, lipoprotein lipase
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
-MF[199]Pancreatic a-amylaseS, 20 mg/L, α-MF[250]Pancreatic triglyceride lipaseS, 75 ml/L, PHO1[251]Papain nitrile hydrataseS, 5 mg/L, α-MF[252]Group-V Phospholipase-A2S, α-MF, active450Placental alkaline phosphatase (PLAP)S, 2 mg/L, PHO1[253]Placental
：也可以-80℃/37℃反复冻融3-5次。 2.7 将肿瘤裂解物加入离心管中，3000rpm，离心10min； 2.8 收取上清，0.22mm滤膜过滤除菌，留样检测蛋白含量及细菌、真菌和支原体； 2.9 -80oC保存备用。 3． CIK细胞的培养及鉴定 3.1 步骤1中获得的PBMC 用无血清培养液调整细胞浓度至2 x 106/ml，置于培养瓶内； 3.2 37℃，5%CO2培养箱中孵育2h，以使单核细胞贴壁; 3.3 收集悬浮细胞，用无血清培养液调整细胞浓度至1-2
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