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- Sino Biological
- 50901-M02H
- 2025年08月12日
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- 文献和实验
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- 规格：
100 µg
蛋白名称：GFR Alpha-3/GFRA3蛋白, GFR Alpha-3/GFRA3 protein
蛋白构建：A DNA sequence encoding the mouse GFRA3 (O35118) (Met 1-Arg 379), was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 80 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asn 29
蛋白分子量：The secreted recombinant mouse GFRA3 Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and has a calculated molecular mass of 66.2 kDa. The apparent molecular mass of rmGFRA3/Fc monomer is approximately 70 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：O35118
蛋白氨基酸序列：Met1-Arg379
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验LABELING ALPHA-ACTININ WITH IATR
with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove
ALPHA FACTOR ARREST AND RELEASE
of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8 M). To release cells from alpha factor arrest, spin down cells and wash twice (using a large volume, dependent on the size of the cell pellet anticipated
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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