- 询价
- ZYMO RESEARCH
- 美国
- D4076
- 2025年12月30日
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- 保存条件：
常温
- 英文名：
Quick-cfDNA Serum & Plasma Kit
- 供应商：
简石生物
HIGHLIGHTS
- High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.
- Highest Yields: Consistently purify > 30% more cfDNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.
DESCRIPTION
Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.
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文献和实验游离DNA可电泳观察，虽然我们做到了，这也是锦上添花的事，因为现有的检测方法完全可以实现更微量核酸的检测工作。血清/血浆游离DNA提取试剂盒 Cat#DK606游离DNA(或称循环DNA，以下简称cfDNA)是一种无细胞状态的、片段化的胞外DNA，存在于血液、滑膜液和脑脊液等体液中。cfDNA在正常人的血液中含量甚微，平均值为13 ng/ml[1]，而当机体在一些特殊状态时(如患有肿瘤、自身免疫性疾病、感染性疾病、中风、心肌梗死及妊娠等)，其含量明显上升，比如恶性肿瘤患者平均值达到180ng/ml
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined from mouse blood samples. For most of these tests, plasma or serum samples are used. Data obtained by these investigations provide indications
Vacuum Protocol: Purification of Viral Nucleic acid from Plasma or Serum
3. Water bath - set to 37°C 实验步骤 1. Transfer 25 μl OB Protease into a sterile microcentrifuge tube. 2. Add 200 μl Plasmid or Serum into the tube and bring the volume up to 225 ul with DEPC treated water.
