我要登录|
免费注册
|
我的丁香通
- 企业机构：
- 成为企业机构
- 个人用户：
- 个人中心
移动端

大家都在搜

大家都在搜

0 人通过求购买到了急需的产品

免费发布求购

点赞

收藏

分享

Vacuum Protocol: Purification of Viral Nucleic acid from Plasma or Serum

互联网2013-11-13

1079

实验试剂

Regents supplied by user

1. Ethanol -approximately 0.3 ml per sample.

实验设备

Equipments supplied by user

1. Tabletop microcentrifuge and sterile 1.5 ml tubes

2. Vacuum Manifold

3. Water bath - set to 37°C

实验步骤

1. Transfer 25 μl OB Protease into a sterile microcentrifuge tube.

2. Add 200 μl Plasmid or Serum into the tube and bring the volume up to 225 ul with DEPC treated water.

3. Add 225 ul Buffer TNA and 5.6 μl Carrier RNA. Mix thoroughly by vortexing.

4. Incubate sample at 45°C for 10 min. Briefly vortex the tube once during incubation.

5. Add 280 ul of isopropanol and mix thoroughly by vortexing. Incubate at room temperature for 5 minutes.

6. Insert the HiBind® MicroElute Viral Column into the vacuum manifold. Carefully apply the lysate to HiBind® MicroElute Viral column. Turn on the vacuum source to draw all liquid through the column. Turn off the vacuum.

Note: If the lysate has difficulty to pass through the column at this stage. Place the column into a collection tube (supplied). Close the lid and centrifuge at 8,000 x g for 5 minutes or until all liquid pass through the column. Place the column into another collection tube (supplied) and continue step 7 of the spin protocol.

7. Pipet 500 ul of Buffer VHB into the column. Turn on the vacuum source to draw all liquid through the column. Turn off the vacuum.

8. Wash the column by pipetting 500 ul of RWB Wash Buffer diluted with ethanol into the column. Turn on the vacuum source to draw all liquid through the column. Turn off the vacuum.

9. Close the lid of HiBind® MicroElute Viral column, remove it from the vacuum manifold. Insert the column into a collection tube (supplied) and centrifuge at 15,000 x g for 2 minute to completely dry the column.

10. Place the column into a sterile 1.5 ml microfuge tube and add 15-50 ul of DEPC-Treated water. Allow tubes to sit for 5 min at room temperature.

11. To elute nucleic acid from the column, centrifuge at 8,000 x g for 1 min. Retain flow-through containing the nucleic acid. Place column into a second 1.5 ml tube.

ad image

ad image

相关产品推荐

SIM SF Expression Medium (For SF9, SF21)(Serum free) | SIM SF 细胞培养基 (用于SF9, SF21细胞)(无血清)

￥450

DKFZ-PSMA-11，4,6,12,19-Tetraazadocosane-1,3,7-tricarboxylic acid, 22-[3-[[[2-[[[5-(2-carboxyethyl)-2-hydroxyphenyl]methyl](carboxymethyl)amin

￥1530

Host Cell Viral Restriction Factor Antibody Sampler Kit

￥500

免疫沉淀protocol

￥600

4S Green Plus Nucleic Acid Stain;S27097--

￥200

相关问答

问

求一份详细的ABE protocol

问

抗体包被的protocol

问

plasma membrane proteins是指什么？它位于何处？

4 回答 638 围观

相关方法

Strep 标签蛋白纯化 protocol

2023-06-02

植物单细胞测序 protocol——水稻花序原生质体制备方法

2023-06-01

推荐阅读

Mitochondrial Nucleic Acid Purification and Analysis

Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions

Determination of Benzoic Acid in Serum or Plasma by Gas Chromatography-Mass Spectrometry (GC/MS)

关于丁香通

公司信息

个人用户

企业机构

无忧采购轻松科研

无忧采购轻松科研

提问

扫一扫

丁香实验小程序二维码

实验小助手

丁香实验公众号二维码

扫码领资料

反馈

TOP

打开小程序