Vacuum Protocol: Purification of Viral Nucleic acid from Plasma or Serum
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实验试剂
1. Ethanol -approximately 0.3 ml per sample.
实验设备
1. Tabletop microcentrifuge and sterile 1.5 ml tubes
实验步骤
1. Transfer 25 μl OB Protease into a sterile microcentrifuge tube.
3. Add 225 ul Buffer TNA and 5.6 μl Carrier RNA. Mix thoroughly by vortexing.
4. Incubate sample at 45°C for 10 min. Briefly vortex the tube once during incubation.



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