重组人白细胞介素-7（rHuIL-7）

Recombinant Human Interleukin 7

品名	rHuIL-7
规格	10μg、 100μg
描述	重组人白介素7是大肠表达系统表达，为一个单链的非糖基化 多肽链蛋白，含154个氨基酸残基，三对二硫键，分子量约为17.5kD，E. coli 表达。
来源	大肠杆菌（E. coli）。
分子量	约为17.5kD。
纯度	≥97%。
内毒素含量	<1EU/µg，用鲎试剂法进行测定。
生物学活性	用细胞增殖的方法进行测定，2E8细胞依赖株，比活性≥2.4×108IU/mg。
配方	本品为无菌冻干粉，缓冲体系为PBS, pH7.4, 0.1%HSA。
复溶	建议开盖前以10,000rpm离心30-60秒，或3,000rpm离心5分钟，再使用0.2ml无菌水复溶。
保存	冻干样品自生产之日起在-20℃到-70℃可以稳定保存36个月。 溶解以后，该细胞因子能在2-8℃的无菌条件下保存一个月，或在-20℃到-70℃的人工除霜的冰箱中保存三个月，而无明显活性损失。 避免反复冻融。

IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B-cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺ and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D) J rearrangement of the T cell receptor β gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross species activity. IL-7 bioactivites are mediated by the binding of IL-7 to functional high affinity receptor complexes. The ligand binding subunit (IL-7R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7R has also been isolated. The γ chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7R and IL-2 Rγ are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.
仅供实验室使用。