- 询价
- Cell Signaling Technology
- 3072
- USA
- 2025年08月26日
- W, IP
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PKR Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Thr451 of human PKR
- 应用范围：
W, IP
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 适应物种：
H
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 74 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PKR Antibody detects endogenous levels of PKR. This antibody does not cross-react with other related proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr451 of human PKR. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha/beta-treated (1000 U/ml for 18 hours), and subsequently untreated or calyculin A-treated ( 0.1 µM for 15 minutes), using PKR Antibody. |
| Background | Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at threonine 446 and threonine 451 in the activation loop. Substitution of threonine 451 to alanine completely inactivates PKR, while a mutant with a threonine 446 to alanine substitution was partially active (3). Recently, activation of PKR has been implicated in the pathologies of neurodegenerative diseases, including Alzheimer disease (4,5).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验PKR in Innate Immunity, Cancer, and Viral Oncolysis
of related cytokines with potent antimicrobial and immuno-modulatory activities. The IFNs exert their effects through the induction of numerous genes, one of which is the double-stranded RNA-dependent protein kinase (PKR), a pivotal antiviral protein
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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