Caspase-9 Antibody (Mouse Spec

9504:

Western Blotting

Caspase-9 Antibody (Mouse Specific) detects endogenous levels of both full length mouse caspase-9 (49 kDa) and the large fragment of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). The antibody does not cross-react other caspases.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Caspase-9 Antibody. p39: caspase-9 cleaved at Asp368. p37: caspase-9 cleaved at Asp353.

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
3. Liu, X. et al. (1996) Cell 86, 147-157.
4. Li, P. et al. (1997) Cell 91, 479-489.
5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
9. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

• Denecker, G. et al. (2001) Yersinia enterocolitica YopP-induced apoptosis of macrophages involves the apoptotic signaling cascade upstream of Bid. J. Biol. Chem. 276 (23), 19706-19714. Applications: Western Blotting
• Zhao, L. et al. (2012) J Biol Chem 287, 1054-65. Applications: Western Blotting

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• 9501 Cleaved Caspase-9 (Asp330) Antibody (Human Specific)
• 9502 Caspase-9 Antibody (Human Specific)
• 9505 Cleaved Caspase-9 (Asp315) Antibody (Human Specific)
• 9507 Cleaved Caspase-9 (Asp353) Antibody (Rat Specific)
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.