Cleaved Caspase-9 (Asp315) Ant

ibody (Human Specific)

价格询价

品牌Cell Signaling Technology
货号
9505
产地USA
更新日期2026年05月28日

应用范围W, IP
宿主Rabbit
适应物种H

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
Cleaved Caspase-9 (Asp315) Antibody (Human Specific)
抗原：
synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9
应用范围：
W, IP
宿主：
Rabbit
供应商：
CST
适应物种：
H
保质期：
详见说明书
级别：
详见MSDS文件
库存：
大量
是否单克隆：
2
保存条件：
-20°c
规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (4 western blots)	产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H	Endogenous	35	Rabbit

Protocols	9505: Immunoprecipitation , Western Blotting
Specificity / Sensitivity	Cleaved Caspase-9 (Asp315) Antibody (Human Specific) detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315. The antibody does not react with full length caspase-9 or any other cleaved caspases.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Cleaved Caspase-9 (Asp315) Antibody (Human Specific) (upper) or Caspase-9 Antibody (Human Specific) #9502 (lower).
Background	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. Liu, X. et al. (1996) Cell 86, 147-157. Li, P. et al. (1997) Cell 91, 479-489. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
Application References	Mitchell, K.O. et al. (2000) Cancer Res 60, 6318-25. Applications: Western Blotting Cheong, J.W. et al. (2003) Clin Cancer Res 9, 5018-27. Applications: Western Blotting Kaiser, C.L. et al. (2008) Hear Res 240, 1-11. Applications: IHC-P (paraffin) Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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