- 询价
- R&D
- 美国/中国
- xy-3523
- 2025年07月11日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody, Purified (SIGNET)详细信息：
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Description 
Purified Monoclonal Antibody against Proliferating Cell Nuclear Antigen (PCNA)
(Formerly Signet Catalog Nos: 523-01, 523-16 L1 Predilute, 523-26 L2 Predilute)Intended Use **Analyte Specific Reagent**
Analytical and performance characteristics are not established.Clone PC10 Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody, Purified (SIGNET)Human IsoType IgG2a Specificity The anti-PCNA monoclonal antibody recognizes a 36kDa protein that is expressed in proliferating cells. Intense nuclear staining is observed in proliferating cells of normal and tumor tissues.
This antibody was generated against rRat PCNA made in the protein A expression vector pR1T2T.
Concentrated Format:
SIG-3523-1000 [1 mL]
Prediluted Formats:
SIG-3523-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems.
SIG-3523-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).Uses Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required.
Incubation: 20-60 minutes at room temperature
Notes Positive tissue (human): Colon or tonsil Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
- Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody, Purified (SIGNET)
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文献和实验of the growth fraction. Proliferating cell nuclear antigen (PCNA) is an endogenous nuclear protein that functions as an accessory protein to DNA polymerase (pol) δ (1 ). Since PCNA is present in all cycling cells, the entire proportion of dividing cells present
Proliferating cell nuclear antigen (PCNA) is a homotrimeric protein adopting a ring structure that may encircle DNA. In this form, PCNA functions as a sliding platform to which different types of catalytic and regulatory proteins are tethered
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
proteins. Taking advantage of the exquisite specificity afforded by each antibody results in a final purified product that binds specifically to that MAb. Using immunoaffinity chromatography, a single antigen can be separated from very complex mixtures (e.g
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