Stemgent® Rabbit anti-Human Vi

Stemgent® Rabbit anti-Human Vimentin Antibody详细信息：
Product Overview

The Stemgent® Rabbit anti-Human Vimentin Antibody is a rabbit monoclonal antibody. This antibody was screened and selected as the best reagent available for visualization of vimentin-expressing cells. It was screened on a human mesenchymal stem cell line and on human fibroblast cells by immuno-cytochemistry. Western blot assay has confirmed that this antibody detects a ~57 kDa protein by SDS/PAGE. Additionally this antibody has been reported to detect vimentin using immunohistochemistry and flow cytometry applications. Three types of cytosolic fibers make up the cytoskeleton: intermediate filaments (IF), microfilaments (for instance, actin) and microtubules. IF function to maintain cell integrity, give shape and motility, as well as to organize cells into specific tissues. Vimentin is a member of the IF family of proteins and is the most ubiquitous IF protein. It is found in many kinds of mesenchymal and epithelial cells, including leukocytes, blood vessel endothelial cells and developing neuronal and astrocytic precursor cells. It is the first IF to be expressed in all early cell types in development and is then replaced by the tissue-specific IFs in non-mesenchymal types as differentiation proceeds. Vimentin is also present in many different tumor types, particularly in those originated from mesenchymal cells. It is used as an immunohistochemical marker for sarcomas and as a marker for mesenchymal cells.

Stemgent® Rabbit anti-Human Vimentin AntibodyProduct Specifications

Stability Stable for 6 months from date of receipt when stored as directed.
Immunogen A synthetic acetylated peptide corresponding to C-terminus of human vimentin was used as an immunogen.
Reactivity Human, Mouse, Rat
Clone EPR3776
Isotype Rabbit IgG
Formulation 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Store undiluted at -20°C. Working aliquots may be stored at 4°C.
Quality Control Tested by immunocytochemistry (ICC) and Western blot (WB) to ensure product quality (Figure 1-3).
Recommended Usage Use antibody at 1:100 dilution in ICC and 1:1,000 dilution in WB. For general protocols for performing ICC and WB in ES cell lines, visit Rabbit anti-Human Vimentin Antibody.
Trademark This product is manufactured under U.S. Patent No. 5,675,063 exclusively hold by Epitomics, Inc.
Stemgent® Rabbit anti-Human Vimentin Antibody
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