Stemgent® Purified Rabbit anti

Stemgent® Purified Rabbit anti-Mouse/Human Oct4 Antibody详细信息：
Product Overview


Octamer-4 (Oct4), a homeodomain transcription factor of the POU family (POU5F1) is involved in regulation of pluripotency during normal development. The expression of Oct4 is associated with an undifferentiated phenotype in embryonic stem (ES) cells, while gene knockdown of Oct4 promotes differentiation. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. Oct4 is one of the four key transcription factors (Oct4, Sox2, c-Myc, and Klf4) used to reprogram mouse and human fibroblasts to a pluripotent state.1-5


Stemgent® Purified Rabbit anti-Mouse/Human Oct4 AntibodyProduct Specifications

Immunogen Synthetic human Oct4 peptide (within residues 300 to the C-terminus) conjugated to KLH
Reactivity Mouse and Human
Clone Polyclonal
Isotype Rabbit IgG
Size 100 μl
Formulation Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide.
Preparation The antibody was purified by immunogen affinity chromatography.
Storage Store undiluted at 4°C. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunocytochemistry and flow cytometry.
Recommended Usage Dilute 1:100 for immunocytochemistry applications and 1:5 for flow cytometry applications.
Technical Documents

Stemgent® Purified Rabbit anti-Mouse/Human Oct4 AntibodySpecifications

09-0023 Specifications Sheet
MSDS

Material Safety Data Sheet
Protocols

General Protocol for Immunocytochemistry
General Protocol for Immunofluorescent Staining of Intracellular Biomarkers Using Flow Cytometry
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