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Ubiquitin Ubi-1 Monclonal Antibody
1
上海信裕
Description | Monoclonal Antibody against Ubiquitin | |
Clone | Ubi-1 | |
Form | Ascites (contains 10mM Sodium Azide) | |
Host | Mouse | |
Species Reactivity | Human, Bovine, Chicken, Drosophila and C. elegans | |
IsoType | IgG1, k light chain | |
Specificity | Ubiquitin Ubi-1 Monclonal Antibody Ubiquitin is a highly conserved globular 76 amino acid protein and has a important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimers disease, the Lewy bodies seen in Parkinsons disease, and Pick bodies found in Picks disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity. Antibody Ubi-1 was raised against purified KLH-conjugated ubiquitin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. Clone Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimers disease. The antibody also works on paraffin embedded material. Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinsons disease and the Pick bodies in Picks disease in formalin fixed tissues. |
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Uses | This antibody is effective in immunoblotting, immunohistochemistry and ELISA. Ubi-1 is excellent for detection of ubiquinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimers disease. | |
Suggested Working Dilution | Ubiquitin Ubi-1 Monclonal Antibody The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
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Notes | * Predicted MW = 8.5kD | |
Storage | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. | |
References | Boutajangout A, Authelet M, Blanchard V, Touchet N, Tremp G, Pradier L, Brion JP. Characterisation of cytoskeletal abnormalities in mice transgenic for wild-type human tau and familial Alzheimers disease mutants of APP and presenilin-1.Neurobiol Dis. 15:47-60, 2004. Wang DS, Bennett DA, Mufson EJ, Mattila P, Cochran E, Dickson DW. Contribution of changes in ubiquitin and myelin basic protein to age-related cognitive decline.Neurosci. Res. 48:93-100, 2004. He CZ, Hays AP. Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J. Neurol. Sci. 217:47-54, 2004. Ungureanu D, Saharinen P, Junttila I, Hilton DJ, Silvennoinen O. Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1. Mol. Cell Biol. 22:3316-3326, 2002. Wirbelauer C, Sutterluty H, Blondel M, Gstaiger M, Peter M, Reymond F, Krek W. The F-box protein Skp2 is a ubiquitylation target of a Cul1-based core ubiquitin ligase complex: evidence for a role of Cul1 in the suppression of Skp2 expression in quiescent fibroblasts. EMBO J. 19:5362-5375, 2000. |
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Warranty/Conditions | Covance products may not be resold or modified for resale without prior written approval. |
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