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- xy-BK126
- 2025年07月08日
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- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Rac1 spec Activation Assay
- 库存：
1
- 供应商：
上海信裕
- Rac1 spec Activation Assay详细信息：
- Product Uses Include
- Rac1 signaling pathway studies
- Rac1 activation assays with primary cells
- Studies of Rac1 activators and inactivators
- Rac1 activation assays with limited material
- High throughput screens for Rac1 activation/inhibition
Rac1 spec Activation AssayIntroduction
The G-LISA series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. The level of activation is measured with luminescence. For a more detailed introduction on G-LISA™ assays and a listing of other available G-LISA™ kits, see our main G-LISA™ page. The Rac1 G-LISA™ Activation Assay (Cat.# BK126) measures the level of GTP-loaded Rac1 protein in cell lysates, this is in contrast to Cat. BK125 which does not distinguish between activated Rac1,2 or 3. For a kit to measure RhoA activation please check webpage BK124.The Rac1 G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples. See this section on our G-LISA™ information resource page.
Rac1 spec Activation Assay
xy-1103xy B7-H1/PDL1/CD274(programmed death ligand 1)程序性死亡配体1（多肽）
xy-0181xy Nociceptin receptor孤菲肽受体（痛敏肽受体）（抗原）
xy-0134xy Nogo-A轴索过度生长抑制因子-A（抗原）
xy-0129xy Nogo R轴索过度生长抑制因子受体（多肽抗原）
xy-0892xy Bad (BCL-xL/BCL-2-associated death promoter)相关死亡促进因子Bad抗原
xy-1304xy Bad (BCL-xL/BCL-2-associated death promoter)相关死亡促进因子Bad抗原
xy-0893xy phospho-BAD(pSer128)磷酸化相关死亡促进因子抗体
xy-1284xy Bak(BCL2 homologous antagonist/killer)Bcl-2同源拮抗剂Bak（多肽）
xy-0156xy NOS-1(bNOS:neuronal NOS:nNOS:Nitric Oxide synthase-1)一氧化氮合成酶-1（抗原）
xy-0162xy NOS-2 (iNOS) Nitric Oxide Synthase,Inducible一氧化氮合成酶-2（抗原）
xy-0911xy Batroxobin蛇毒巴曲酶抗原
xy-0163xy NOS-3 (eNOS) endotheli cell NOS一氧化氮合成酶-3（抗原）
xy-0071xy NPY ( Neuropeptide Y)神经肽 Y（抗原）
xy-0222xy NR2B (Glutamate receptor)谷氨酸受体（抗原）
xy-0160xy NT-3神经生长因子-3（抗原）
xy-0158xy NT-4,NT-5神经生长因子4/5（抗原）
xy-0073xy NTN (Neurturin)神经生长因子（抗原）
xy-1336xy Bcl-xL(human, mo, rat, bovine, dog, pig, sheep, horse)Bcl-xL蛋白抗原
xy-1337xy phospha-Bcl-xL (pSer62) peptide (human, mo, rat, bovine, dog, pig, sheep, horse)磷酸化(Ser62)Bcl-xL蛋白抗原
xy-0986xy BCHE(butyrylcholinesterase)NT丁酰胆碱脂酶抗原(N端)
xy-0987xy BCHE(butyrylcholinesterase)CT丁酰胆碱脂酶抗原(C端)
xy-0178xy Nurr1核受体相关因子-1（抗原）
xy-0372xy NT5C2胞浆-5′-核苷酸酶-Ⅱ
xy-1353xy BECN1(Beclin1)一种抑癌基因（多肽）
xy-0240xy OGP (Ostegenic Growth Peptide)成骨生长肽 (骨生长激素肽)(蛋白多肽)
xy-0019xy OPN骨桥蛋白（多肽抗原）
xy-2431xy BAFF/CD257(B cell activating factor)TNF家族B细胞激活因子抗原
xy-0033xy P53肿瘤抑制基因（抗原）
xy-0190xy PACAP-27/38腺苷酸环化酶激活肽-27/38（抗原）
xy-0200xy PACAP-38腺苷酸环化酶激活肽-38（抗原）
xy-1153xy Bid (BH3 interacting domain death agonist)BH3结构域凋亡诱导蛋白抗原
xy-2357xy PACAP(6-38 Peptide)腺苷酸环化酶激活肽6-38
xy-0197xy PACAP/VIP receptor腺苷酸环化酶激活肽/血管活性肠肽受体（抗原）
xy-0198xy PACAP receptor-I腺苷酸环化酶激活肽受体（抗原）
xy-0318xy PADI4 (HL-60 PAD)(Protein-arginine deiminase type IV，PADI 4，PAD I4 )关节炎相关基因
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho
Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
, one needs to introduce these promoter sequences into specific cells, and by linking them to an appropriate reporter gene, one can estimate promoter activity by an increase in reporter gene activity either by enzymatic assay or mRNA expression. Various
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