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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Rac1,2,3 G-LISA Activation Assay
- 库存：
1
- 供应商：
上海信裕
- Rac1,2,3 G-LISA Activation Assay详细信息：
- Product Uses Include
- Rac signaling pathway studies
- Rac activation assays with primary cells
- Studies of Rac activators and inactivators
- Rac activation assays with limited material
- High throughput screens for Rac activation
Rac1,2,3 G-LISA Activation AssayIntroduction
The G-LISA series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. For a more detailed introduction on G-LISA™ assays and a listing of other available G-LISA™ kits, see our main G-LISA™ page. The Rac1,2,3 G-LISA™ Activation Assay measures the entire level of GTP-loaded Rac1,2 and 3 protein in cell lysates, this is in contrast to Cat.# BK126 which measures only Rac1 activation levels. The level of activation is measured with absorbance at 490nm. For a kit to measure RhoA activation please check webpage BK124.The Rac G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples. See this section on our G-LISA™ information resource page.
Rac1,2,3 G-LISA Activation Assay
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Activation of MAPKs by G Protein-Coupled Receptors
-triphosphate (GTP) bound to the α-subunit on receptor activation. Consequently, Gα- and Gβγ-subunits stimulate effector molecules, including adenylyl and guanylyl cyclases, phosphodiesterases, phospholipase A2 (PLA2 ) and PLC, and phosphatidylinositol-3 kinases
[35S]GTPS Binding as an Index of Total G-Protein and G-Subtype-Specific Activation by GPCRs
On activation, G-protein-coupled receptors (GPCRs) exert many of their cellular actions through �promoting guanine nucleotide exchange on Gα subunits of heterotrimeric G proteins to release Gα-GTP and free βγ-subunits. In membrane
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