Rho Activation Assay Kit

Product Uses Include

Rho Activation Assay Kit Introduction
The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors RhoA protein activation. The assay uses the Rho binding domain (RBD) of the Rho effector protein, Rhotekin. The RBD motif has been shown to bind specifically to the GTP-bound form of RhoA. The fact that the RBD region of Rhotekin has a high affinity for GTP-RhoA and that Rhotekin binding results in a significantly reduced intrinsic and catalytic rate of GTP hydrolysis make it an ideal tool for affinity purification of GTP-RhoA from cell lysates. The Rhotekin-RBD protein supplied in this kit contains Rhotekin residues 7-89 and is in the form of a GST fusion protein, which allows one to "pull-down" the Rhotekin-RBD/Rho-GTP complex with brightly colored glutathione affinity beads. The assay therefore provides a simple means of quantitating RhoA activation in cells. The amount of activated RhoA is determined by a western blot using a RhoA specific antibody.
Rho Activation Assay Kit
xy-1004xy ACE2(Angiotensin converting enzyme 2)血管紧张素转换酶2抗原
xy-0044xy BNP脑钠素（多肽抗原）
xyb-0044xy BNP/Biotin生物素化脑钠素多肽
xy-0324xy C5过敏毒素C5（补体C5）抗原
xy-0091xy CA125卵巢癌抗原
xy-0043xy CAD(Caspase-Activated Deoxyribonuclease)Caspase 激活的脱氧核糖核酸酶（抗原）
xy-3550xy MYOG肌红蛋白抗原
xy-0095xy Calponin 1钙调节蛋白-1（抗原）
xy-3679xy COMP软骨寡聚基质蛋白抗原
xy-0169xy Caspase-1 (ICE; CASP-1；P45)天冬氨酸-胱氨酸特异性蛋白酶家族（抗原）
xy-0081xy Caspase-3 (CPP32)半胱胺酸蛋白酶蛋白-3（抗原）
xy-0151xy Caspase-6 (CT)半胱胺酸蛋白酶蛋白-6（抗原）
xy-0049xy Caspase-9白介素1-β转化酶样凋亡蛋白酶6（抗原）
xy-0050xy Caspase-9 白介素1-β转化酶样凋亡蛋白酶6（抗原）
xy-0092xy Caspase-10 半胱胺酸蛋白酶-10（抗原）
xy-0053xy Caspase-13 半胱胺酸蛋白酶蛋白-13（抗原）
xy-0079xy CD19(B-lymphocyte antigen CD19)CD19
xy-2167xy DRADA/ADAR1(Double-stranded RNA-specific adenosine deaminase)N terminal双链RNA腺苷酸脱氨基酶抗原（N端）
xy-2168xy DRADA/ADAR1(Double-stranded RNA-specific adenosine deaminase)双链RNA腺苷酸脱氨基酶抗原
xy-4593xy MMP9基质金属蛋白酶9
xy-4622xy Mycobacterium bovis 牛结核杆菌菌体蛋白
xy-4687xy AMH/MISMuellerian抑制因子抗原
xy-2231xy AEG-1 peptideAEG-1抗原
xy-0195xy CD31 (PECAM-1)血小板内皮细胞黏附分子-1（抗原）
xy-1164xy ADFP/ADRP/adipophilin 脂肪组织分化相关蛋白抗原
xy-0298xy Immunoglobuin binding protein-1(CD79A)IGBP-1免疫球蛋白结合蛋白-1
xy-4770xy CD133造血干细胞抗原CD133
xy-0209xy CD133造血干细胞抗原（多肽抗原）
xy-4813xy H5N1-H5禽流感H5亚型全病毒
xy-4814xy Newcastle disease virus鸡新城疫疫苗（鸡瘟4型混合病毒）
xy-4938xy SDF-1/CXCL12基质细胞衍生因子1
Rho Activation Assay Kitxy-3681xy ADCY1 peptide腺苷酸环化酶1多肽抗原
xy-0125xy c-erbB-2蛋白c-erbB-2蛋白（抗原）
xy-5109xy HCLS1造血干细胞特异性蛋白