Rac Activation Assay Kit

Product Uses Include

Rac Activation Assay Kit Introduction
The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. The PAK-PBD protein supplied in this kit corresponds to residues 67-150. This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Rac (or GTP-Cdc42) complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Rac or Cdc42 activation in cells. The amount of activated Rac is determined by a Western blot using a Rac-specific antibody.
Rac Activation Assay Kit
xy-0154xy GAP-43 ( Growth associated protein-43 )生长相关蛋白-43（抗原）
xy-0010xy Gastrin胃泌素（抗原）
xy-0840xy AMACR/P504S(alpha-methylacyl-CoA racemase)α－甲基酰基辅酶A消旋酶抗原
xy-0199xy GFAP(Glial Fibrillary Acidic Protein)胶质纤维酸性蛋白（抗原）
xy-1116xy AMPK Beta1 (AMP-activated Protein Kinase beta-1)腺苷单磷酸活化蛋白激酶β1抗原
xy-0424xy MMP-1基质金属蛋白酶-1
xy-0412xy MMP-2基质金属蛋白酶-2(多肽抗原)
xy-0413xy MMP-3(matrix metalloproteinase-3/Transin-1/SL-1/Stromelysin-1 precursor)基质金属蛋白酶-3(抗原)
xy-0423xy MMP-7(Matrilysin/matrix metalloproteinases-7)基质金属蛋白酶-7(抗原)
xy-0414xy MMP-14(Matrix metalloproteinase-14)金属基质蛋白酶-14
xy-0201xy GFRAlpha-1(GDNF family receptor alpha-1)GDNF家族受体α-1（抗原）
xy-0587xy Ang- II (Angiotensin II )血管紧张素II抗原
xy-0351xy GLUT2(Glucose transporter type 2)葡萄糖转运蛋白2（抗原）
xy-2040xy ANP(atrial natriuretic peptide)心钠肽（心钠素）抗原
xy-0168xy GFRA4 ( Persephin receptor ) (GFR receptor alpha 4)GDNF家族受体α-4（抗原）
xy-0205xy GHRF (GHRH)生长激素释放激素（因子）（抗原）
xy-0800xy ANGPTL1/ANG-1 (Angiopoietin-like Protein 1)血管位蛋白样1/血管生成素样蛋白-1抗原
xy-0384xy GLUT4(Glucose transporter type 4)葡萄糖转运蛋白4（抗原）
xy-0098xy GIP (Gastric Inhibitory polypeptide)胃泌素抑制肽（抗原）
xy-0537xy ANGPTL2/ANG-2 (Angiopoietin-like Protein 2)血管位蛋白样2/血管生成素样蛋白-2（多肽）
xy-0208xy GLP-2(Glucagon-like peptide-2)胰高血糖素样肽-2（多肽抗原）
xy-0194xy GRP94 (gp96)94kDa 糖调节蛋白（抗原）
xy-1171xy Annexin A膜粘连蛋白 A抗原
xy-0348xy G Protein(Guanine nucleotide-binding regulatory protein)G蛋白（鸟苷酸结合蛋白）（抗原）
xy-0252xy GR (Glucocorticoid receptor)糖皮质激素受体 多肽
xy-0576xy Annexin II膜粘连蛋白-2抗原
xy-0341xy CKR-L2( G protein-coupled receptor-2 )G蛋白偶联受体-2(抗原)
xy-0136xy GTP-CH-1三磷酸鸟苷环水解酶（抗原）
xy-0344xy H5N1-M2(Avian influenza Matrix Protein-2)H5N1流感病毒M2型（抗原）
xy-0221xy HCV-Core丙型肝炎病毒-C区（多肽）
xy-0218xy HCV-NS1丙型肝炎病毒-NS1（抗原）
xy-0219xy HCV-NS3丙型肝炎病毒-NS3（抗原）
xy-0213xy HCV-NS4a丙型肝炎病毒-NS4a（抗原）
xy-0212xy HEV戊型肝炎病毒（抗原）
xy-0338xy HGV(Hepatitis G vivus)庚型肝炎病毒（抗原）
Rac Activation Assay Kitxy-0287xy HisX6聚组氨酸标签蛋白
xy-0241xy HIV gp120艾滋病病毒（抗原）