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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃保存
- 保质期:
6个月
- 英文名:
X-rhodamine tubulin, lyophilized
- 库存:
1
- 供应商:
上海信裕
- X-rhodamine tubulin, lyophilized详细信息:
- Product Uses Include
- Laser based applications
- Monitoring microtubule dynamcs in living cells by microinjection
- Speckle microscopy
- Formation of fluorescent microtubules
- Microscopy studies of MAP and microtubule associated motor activities
- Nanotechnology
X-rhodamine tubulin, lyophilizedMaterial
Bovine brain tubulin (>99% pure, see Cat. # TL238) has been modified to contain covalently linked rhodamine at random surface lysines. An activated ester of X-rhodamine (ROX) was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 70,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. X-rhodamine labeled tubulin can be detected using a filter set of 560-580 nm excitation and 605-620 emission. X-rhodamine tubulin is in a versatile, stable and easily shipped format. When dissolved in G-PEM, it is ready for micro-injection, when dissolved in G-PEM with 5% glycerol, it is ready for in vitro polymerization.Cytoskeleton, Inc. also offers rhodamine and fluorescein labeled tubulin of the same quality (for example Cat.# TL331M and T332M).
X-rhodamine tubulin, lyophilized
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X-rhodamine tubulin, lyophilizedxy-3251R Phospho-5-Lipoxygenase(Ser271)磷酸化5-脂氧合酶抗体
xy-3252R Phospho-5-Lipoxygenase(Ser663)磷酸化5-脂氧合酶抗体
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文献和实验the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold room, discard the supernatant. Add 1ml of column buffer containing 0.5 mM GTP to each tube
the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin
Thaw tubulin Adjust solution to a final buffer concentration equivalent of glycerol PB Incubate at 37 o C for 30 min. Layer tubulin onto cushions of BRB80 containing 60% (v/v) glycerol
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