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X-rhodamine tubulin, lyophiliz

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  • 询价
  • ATCC
  • 美国/中国
  • xy-TL334M
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      4℃保存

    • 保质期

      6个月

    • 英文名

      X-rhodamine tubulin, lyophilized

    • 库存

      1

    • 供应商

      上海信裕

    • X-rhodamine tubulin, lyophilized详细信息:
    • Product Uses Include
      • Laser based applications
      • Monitoring microtubule dynamcs in living cells by microinjection
      • Speckle microscopy
      • Formation of fluorescent microtubules
      • Microscopy studies of MAP and microtubule associated motor activities
      • Nanotechnology
      X-rhodamine tubulin, lyophilizedMaterial
      Bovine brain tubulin (>99% pure, see Cat. # TL238) has been modified to contain covalently linked rhodamine at random surface lysines. An activated ester of X-rhodamine (ROX) was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 70,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. X-rhodamine labeled tubulin can be detected using a filter set of 560-580 nm excitation and 605-620 emission. X-rhodamine tubulin is in a versatile, stable and easily shipped format. When dissolved in G-PEM, it is ready for micro-injection, when dissolved in G-PEM with 5% glycerol, it is ready for in vitro polymerization.
      Cytoskeleton, Inc. also offers rhodamine and fluorescein labeled tubulin of the same quality (for example Cat.# TL331M and T332M).
      X-rhodamine tubulin, lyophilized
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    图标文献和实验
    相关实验
    • Preparation of tubulin

      the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold room, discard the supernatant.  Add 1ml of column buffer containing 0.5 mM GTP to each tube

    • Tubulin Preparat

      the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin

    • Cycling Tubulin

      Thaw tubulin Adjust solution to a final buffer concentration equivalent of glycerol PB Incubate at 37 o C for 30 min. Layer tubulin onto cushions of BRB80 containing 60% (v/v) glycerol

    图标技术资料

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