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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃保存
- 保质期:
6个月
- 英文名:
Rhodamine tubulin, lyophilized
- 库存:
1
- 供应商:
上海信裕
- Rhodamine tubulin, lyophilized详细信息:
- Product Uses Include
- Monitoring microtubule dynamcs in living cells by microinjection (TL331M & T331M)
- Formation of fluorescent microtubules (TL331M, T331M & T331)
- Microscopy studies of MAP and microtubule associated motor activities (TL331M, T331M & T331)
- Nanotechnology (TL331M, T331M & T331)
Rhodamine tubulin, lyophilizedMaterial
Bovine brain tubulin (>99% pure, see Cat. # TL238) has been modified to contain covalently linked rhodamine at random surface lysines. An activated ester of the fluorochrome [(5-(and 6)-carboxytetramethylrhodamine succinimidyl ester] was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 70,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. Rhodamine labeled tubulin can be detected using a filter set of 535 nm excitation and 585 emission.
Rhodamine tubulin, lyophilized
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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
to tubulin. We have used it successfully to derivatize tubulin with succinimidyl esters of biotin, digoxigenin, and a wide range of fluorochromes such as tetramethylrhodamine, X-rhodamine, fluorescein, Oregon Green, Cy3, Cy5 and C2CF (bis-caged
Labeling Tubulin and Quantifying Labeling Stoichiometry
of biotin, digoxigenin, and a wide range of fluorochromes such as tetramethylrhodamine, X-rhodamine, fluorescein, Oregon Green, Cy3, Cy5 and C2CF (bis-caged carboxyfluorescein). The procedure involves labeling polymeric tubulin, thereby protecting residues
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
and sensitive to changes in both the chemical and mechanical environment. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins
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