PIP3 Mass ELISA K-2500s Echelo

Description: The PIP₃ Mass ELISA measures the amount of PI(3,4,5)P₃ extracted from cells by means of a competitive ELISA, (96 well format).

PIP3 Mass ELISA K-2500s EchelonClass I Phosphoinositide 3-kinases (PI3-Kinase, PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PtdIns(4,5)P₂ at the 3 position hydroxyl of the inositol ring producing PtdIns(3,4,5)P₃ (PIP₃). PIP₃ has shown to be an important lipid second messenger in multiple cell signaling pathways such as proliferation, metabolism, calcium release, and survival. Due to these interesting biological roles, PI3-K and PIP₃ have become attractive targets for research and drug development.

The PIP₃ Mass ELISA Kit allows for the determination of PI3-K activity by quantifying the amount of PIP₃ found in cells. After following a non-radioactive lipid extraction protocol, the PIP₃ samples obtained from millions of cells are detected on a competitive ELISA. The cellular PIP₃ samples are mixed and incubated with a PIP₃Detector protein, then added to a PIP₃-coated microplate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the PIP3 protein binding to the plate. The assay is sensitive to 0.04 pmol PIP₃. The colorimetric signal is inversely proportional to the amount of PIP₃ obtained.

PIP3 Mass ELISA K-2500s EchelonFormats

• 96-well Competitive ELISA

Storage

• 2-8°C, and -20°C

Reference

• Nonradioactive Quantification of Phosphoinositide 3-kinase (PI3K) Products from Breast Cancer Cell Lines andQuantification of PI3-Kinase Products from Breast Cancer Cell Lines
• PIP3 Mass ELISA K-2500s Echelon
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